DNA DAMAGE - DiVA Portal

Ku70 serine 155 medierar aurora b-hämning och aktivering av DNA

2013-07-01 There was no correlation between the transcript levels of Ku80 and LUC luminescence derived from T‐DNA stable transformation in KD‐OsKu80 rice calli (Figs 1b, 2d,e). It has been shown that the Ku70/80 protein functions as a heterodimer to repair DSBs, with the two constituents stabilizing each other (Smider et al., 1994). 2016-07-08 DNA-PKcs functions both within and outside of the damage response to effectively design therapeutic strategies. Ku70 and Ku80 (also called Ku86) are encoded by the XRCC6 and XRCC5 genes, respectively, in humans, and have a strong afﬁ nity for free ends of DNA ( 21 ).

To distinguish between these possi-bilities, the Ku70 and Ku80 mutations were crossed together to generate Ku70, Ku80, and double Ku70 forms a heterodimer with Ku80, called Ku that is well known for repairing DNA double-strand breaks through non-homologous end joining. As a result, deletion of either causes a very similar The Ku70–Ku80 heterodimer of other species binds to the termini of double‐stranded DNA as the initial step in DSB repair. To determine whether the AtKu70–AtKu80 heterodimer also binds to DNA fragments, we performed EMSA analysis with the recombinant proteins produced in E. coli . Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96].

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might function as part of a telomeric length sensing system protecting chromosomal termini from nucleolytic attack, as was shown in yeast (Boulton and Jackson, 1996). Recent gene knockout experiments in mice pointed out yet another function of Ku. As expected, both Ku70 and Ku80 … The Ku70–Ku80 heterodimer of other species binds to the termini of double‐stranded DNA as the initial step in DSB repair. To determine whether the AtKu70–AtKu80 heterodimer also binds to DNA fragments, we performed EMSA analysis with the recombinant proteins produced in E. coli .

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To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine embryonic stem cell line which lacks Ku70 expression. We find that the Ku70 -/- cells produce no detectable Ku70 and very little Ku80, suggesting a direct interrelationship between their levels. Both Ku70 and Ku80 therefore contain monopartite NLSs, and sequences outside the basic cluster make favorable interactions with Impα, suggesting that this may be a general feature in monopartite NLSs. We show that the Ku70 NLS has a higher affinity for Impα than the Ku80 NLS, consistent with more extensive interactions in its N-terminal region.

Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V(D)J recombination product formation defects.
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Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Ku80 is a protein that, in humans, is encoded by the XRCC5 gene.

Ku70/80 was incubated with PARP-1 in the presence of sonicated DNA and NAD + (lanes 1 and 3) or buffer only (lanes 2 and 4). Cell Reports Article PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF Satish K. Tadi,1 Carine Tellier-Lebe`gue,2 Cle´ment Nemoz,2 Pascal Drevet,2 Ste´phane Audebert,1 Sunetra Roy,3,4 1 Dec 2008 The NHEJ pathway can be inactivated by targeting the Ku70 or Ku80 Phylogenetic tree of KU70 and KU80 fungal proteins whose function in 14 Sep 2020 of the Ku70/80 heterodimer in vitro. We further defined the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and They also have significantly diminished levels of Ku80 protein. Therefore, Ku70's role in DNA repair may be linked to the known biochemical features of Ku, InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites.
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DNA repair pathways and the effect of radiotherapy in breast cancer

2564 BE — CRISPR/Cas9-mediated loss of FGF5 function increases wool staple we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase IV By agreement with the company, the product's function will not be exposed in the report. of CUX1 with some of these proteins: REV1, PARP1 and Ku70/Ku80. In this thesis, we have studied factors that affect tumour cell resistance to ionising radiation.

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Lund University Bioimaging Center - Research Outputs - Lund

We further defined the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and They also have significantly diminished levels of Ku80 protein. Therefore, Ku70's role in DNA repair may be linked to the known biochemical features of Ku, InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures 5 Mar 2021 Plays a role in the regulation of DNA virus-mediated innate immune response by Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.